1XFD
Structure of a human A-type Potassium Channel Accelerating factor DPPX, a member of the dipeptidyl aminopeptidase family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2004-01-25 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.980, 170.230, 159.316 |
| Unit cell angles | 90.00, 92.11, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.2481 |
| Rwork | 0.248 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL BASED ON PDB ENTRY 1N1M |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.098 |
| Number of reflections | 76240 |
| <I/σ(I)> | 15 |
| Completeness [%] | 90.6 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.25 | 295 | MES, NaCl, PEG MME 2000, magnesium chloride, pH 6.25, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
