1XF9
Structure of NBD1 from murine CFTR- F508S mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-04 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 170.449, 170.449, 109.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.400 - 2.700 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.25300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1r0x heterogens removed |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.400 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 44242 | |
| <I/σ(I)> | 22.9 | 2.3 |
| Completeness [%] | 98.8 | 95.6 |
| Redundancy | 7.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Sodium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
