1XF1
Structure of C5a peptidase- a key virulence factor from Streptococcus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 114.696, 75.113, 132.391 |
| Unit cell angles | 90.00, 104.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.229 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 171036 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 291 | pH 4.5, VAPOR DIFFUSION, , temperature 291K |
