1XDN
High resolution crystal structure of an editosome enzyme from trypanosoma brucei: RNA editing ligase 1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97885, 0.97899, 0.96112 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.911, 58.579, 52.984 |
| Unit cell angles | 90.00, 100.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.12923 |
| Rwork | 0.128 |
| R-free | 0.14805 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.064 | 0.064 |
| Number of reflections | 95711 | |
| <I/σ(I)> | 33.8 | 33.8 |
| Completeness [%] | 86.8 | 86.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 3350, magnesium chloride, Tris, ATP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
