1XD6
Crystal structures of novel monomeric monocot mannose-binding lectins from Gastrodia elata
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 2001-04-15 |
| Detector | WEISSENBERG |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 78.070, 97.480, 36.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.930 - 2.000 |
| R-factor | 0.20267 |
| Rwork | 0.199 |
| R-free | 0.23829 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.930 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 9217 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 95.5 | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | ammonium sulfate, MPD, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
