1XCG

Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Detector technology	CCD
Collection date	2004-04-18
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9790
Spacegroup name	P 1 21 1
Unit cell lengths	89.236, 118.782, 90.130
Unit cell angles	90.00, 114.34, 90.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.235
Rwork	0.224
R-free	0.28100
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.268
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.500
Number of reflections	57314
Completeness [%]	96.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.2	298	PEG, Hepes, pH 7.2, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	7.2	298	PEG, Hepes, pH 7.2, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	7.2	298	PEG, Hepes, pH 7.2, VAPOR DIFFUSION, temperature 298K
1	VAPOR DIFFUSION	7.2	298	PEG, Hepes, pH 7.2, VAPOR DIFFUSION, temperature 298K