1XC6

Native Structure Of Beta-Galactosidase from Penicillium sp. in complex with Galactose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE D03B-MX1
Synchrotron site	LNLS
Beamline	D03B-MX1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-01-01
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 43
Unit cell lengths	110.960, 110.960, 161.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.400 - 2.100
Rwork	0.171
R-free	0.18590
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.338
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.400	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.107	0.481
Number of reflections	224036
<I/σ(I)>	12.5	3.1
Completeness [%]	99.9	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	291	15% PEG 8000, 50 MM Sodium Phosphate (Iodide ions were incorporated into the crystal by Quick Cryo Soaking technique at room temperature), pH 4.0, Vapor Diffusion, Hanging Drop, temperature 291K