1XAW

crystal structure of the cytoplasmic distal C-terminal domain of occludin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9
Spacegroup name	C 2 2 21
Unit cell lengths	46.960, 109.570, 48.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.730 - 1.450
R-factor	0.265
Rwork	0.261
R-free	0.28400
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	22041
Completeness [%]	99.2	98.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	295	amonium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 295.0K
1	VAPOR DIFFUSION	5.6	295	amonium sulfate, pH 5.6, VAPOR DIFFUSION, temperature 295.0K