1XA1
Crystal structure of the sensor domain of BlaR1 from Staphylococcus aureus in its apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.10000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.876, 104.893, 90.273 |
| Unit cell angles | 90.00, 107.79, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.18824 |
| Rwork | 0.186 |
| R-free | 0.22710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | benzylpenicillin-acylated BlaR1 C-terminal domain PDB entry 1XA7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.296 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 95274 | |
| Completeness [%] | 97.3 | 78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 291 | 18-23% PEG 3350, 0.2M NaH2PO4, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
