1XA0
Crystal Structure of MCSG Target APC35536 from Bacillus stearothermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 99.8 |
Detector technology | CCD |
Collection date | 2004-03-18 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9790 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 156.236, 156.236, 68.569 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.21836 |
Rwork | 0.212 |
R-free | 0.26931 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.420 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.085 | 0.575 |
Number of reflections | 28389 | |
<I/σ(I)> | 5.1 | |
Completeness [%] | 99.9 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.3 | 298 | 160mM Ammonium sulfate, pH 8.3, VAPOR DIFFUSION, temperature 298K |