1X9R
Umecyanin from Horse Raddish- Crystal Structure of the oxidised form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.542 |
Spacegroup name | P 65 |
Unit cell lengths | 92.690, 92.690, 47.310 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.660 - 1.900 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jer |
RMSD bond length | 0.017 |
RMSD bond angle | 2.830 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.290 | 1.960 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.269 |
Number of reflections | 18450 | |
<I/σ(I)> | 45 | 16 |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 8.6 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | PEG3350, Bicine, Magnesium chloride, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |