1X9Q
4m5.3 anti-fluorescein single chain antibody fragment (scFv)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9840 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.780, 57.450, 79.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.750 - 1.500 |
| R-factor | 0.1929 |
| Rwork | 0.165 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flr |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 40648 |
| Completeness [%] | 98.8 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 277 | 0.15 M Sodium Acetate, 0.1 M Ammonium Acetate, 20% PEG 4000, 10% Glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
