1X9G

PUTATIVE MAR1 RIBONUCLEASE FROM LEISHMANIA DONOVANI

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Detector technology	CCD
Collection date	2004-04-27
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9794, 0.9793, 0.8919
Spacegroup name	I 4 3 2
Unit cell lengths	147.438, 147.438, 147.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.406 - 2.410
R-factor	0.22969
Rwork	0.225
R-free	0.27567
Structure solution method	MAD
RMSD bond length	0.020
RMSD bond angle	1.970
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.06)
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.400	50.000	2.470
High resolution limit [Å]	2.400	5.490	2.400
Rmerge	0.075	0.033	0.918
Number of reflections	10935
Completeness [%]		98.4	100
Redundancy	20.8	19	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.7	290	0.5 ul protein 10 mg/ml, 5% DMSO, 2.5% glycerol, 250 mM NaCl, 10mM HEPES pH 7.5 0.5 ul crystallization buffer 18% PEG 1000, 50 mM MES pH 5.6, 50 mM K3PO4, pH 5.7, VAPOR DIFFUSION, SITTING DROP, temperature 290K