1X9F

Hemoglobin Dodecamer from Lumbricus Erythrocruorin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200H
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	139.225, 172.071, 202.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	105.410 - 2.600
R-factor	0.21481
Rwork	0.213
R-free	0.24615
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3.8 Structure of the whole molecule
RMSD bond length	0.009
RMSD bond angle	1.875
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	105.410
High resolution limit [Å]	2.600
Number of reflections	74920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	281	2.8M Na phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 281.0K
1	VAPOR DIFFUSION, HANGING DROP	6.8	281	2.8M Na phosphate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 281.0K