1X8X

Tyrosyl t-RNA Synthetase from E.coli Complexed with Tyrosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32B2
Synchrotron site	SPring-8
Beamline	BL32B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	82.820, 82.820, 93.490
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.410 - 2.000
R-factor	0.194
Rwork	0.191
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.100
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNX (2002)
Refinement software	CNX (2002)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.726	2.110
High resolution limit [Å]	2.000	2.000
Number of reflections	25543
<I/σ(I)>	8.7	3.9
Completeness [%]	99.9	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	ammonium sulfate, PEG400, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K