1X8K
Crystal structure of retinol dehydratase in complex with anhydroretinol and inactive cofactor PAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.286, 66.661, 84.464 |
| Unit cell angles | 90.00, 110.66, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.750 |
| R-factor | 0.19905 |
| Rwork | 0.194 |
| R-free | 0.25506 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.525 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 20545 | |
| <I/σ(I)> | 7.8 | 1.9 |
| Completeness [%] | 96.8 | 81.5 |
| Redundancy | 3.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 289 | 7.5% PEG4000, 0.1M Na Hepes, 0.05M calcium chloride, 4% glycerol, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
