1X8I
Crystal Structure of the Zinc Carbapenemase CphA in Complex with the Antibiotic Biapenem
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-18 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.836, 101.513, 117.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.500 - 1.900 |
| R-factor | 0.15311 |
| Rwork | 0.151 |
| R-free | 0.18308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CphA (N220G) mutant |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.748 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.500 | 1.960 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.290 |
| Number of reflections | 20636 | |
| <I/σ(I)> | 3.7 | 2.1 |
| Completeness [%] | 93.1 | 99.9 |
| Redundancy | 5.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 280 | PEG, AS, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
