1X8G
Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979469 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 42.628, 101.205, 118.239 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.18401 |
| Rwork | 0.183 |
| R-free | 0.20118 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CphA (N220G mutant) |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.629 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.722 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.123 | 0.312 |
| Number of reflections | 27567 | |
| <I/σ(I)> | 3.9 | 2.8 |
| Completeness [%] | 94.7 | 94.7 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 278 | PEG, AS, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
