1X79
Crystal structure of human GGA1 GAT domain complexed with the GAT-binding domain of Rabaptin5
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2003-07-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418,0.95667,0.97938,0.97952 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 155.222, 155.222, 53.052 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.810 - 2.410 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.405 |
| Number of reflections | 28214 | |
| <I/σ(I)> | 30.1 | 2.3 |
| Completeness [%] | 97.2 | 77.3 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | AMMONIUM SULFATE, TRIS, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
