1X6U
KDO8P synthase in it's binary complex with the product KDO8P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.934 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 118.530, 118.530, 118.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1phw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.307 | |
| Number of reflections | 6950 | |
| <I/σ(I)> | 13.1 | 5.3 |
| Completeness [%] | 90.7 | 99.9 |
| Redundancy | 6.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | PEG4000, Glycerol, Tris-HCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
