1X56
Crystal structure of asparaginyl-tRNA synthetase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-30 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.122, 68.597, 75.641 |
| Unit cell angles | 90.00, 117.05, 90.00 |
Refinement procedure
| Resolution | 34.300 - 1.980 |
| R-factor | 0.17865 |
| Rwork | 0.177 |
| R-free | 0.21158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8a |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 39362 | |
| Completeness [%] | 98.7 | 91.6 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
