1X54
Crystal structure of asparaginyl-tRNA synthetase from Pyrococcus horikoshii complexed with asparaginyl-adenylate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.071, 68.221, 75.706 |
| Unit cell angles | 90.00, 117.18, 90.00 |
Refinement procedure
| Resolution | 30.290 - 1.450 |
| R-factor | 0.16903 |
| Rwork | 0.168 |
| R-free | 0.19157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.296 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 97438 | |
| Completeness [%] | 96.1 | 78.1 |
| Redundancy | 4.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
