1X31

Crystal Structure of Heterotetrameric Sarcosine Oxidase from Corynebacterium sp. U-96

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00817
Spacegroup name	P 65 2 2
Unit cell lengths	199.112, 199.112, 197.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.060 - 2.150
R-factor	0.188
Rwork	0.188
R-free	0.23200
Structure solution method	MAD
RMSD bond length	0.791
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.060	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.092	0.319
Number of reflections	124207
Completeness [%]	99.8	98.3
Redundancy	10.3	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	0.1M Tris-HCl, 1.9M ammonium sulfate, 10mM CuSO4, pH 8.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8.5	293	0.1M Tris-HCl, 1.9M ammonium sulfate, 10mM CuSO4, pH 8.5, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION	8.5	293	0.1M Tris-HCl, 1.9M ammonium sulfate, 10mM CuSO4, pH 8.5, VAPOR DIFFUSION, temperature 293K