1X23
Crystal structure of ubch5c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.998, 44.334, 97.584 |
| Unit cell angles | 90.00, 93.36, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.20376 |
| Rwork | 0.199 |
| R-free | 0.28222 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qcq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.467 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.249 |
| Number of reflections | 46077 | |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 93.9 | 83.8 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 0.1M HEPES-Na, 10% iso-propanol, 20% PEG4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
