1X1R
Crystal structure of M-Ras in complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-05-25 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.899, 70.668, 43.422 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.440 - 1.300 |
R-factor | 0.18269 |
Rwork | 0.182 |
R-free | 0.20003 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q21 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.293 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.700 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.064 | 0.286 |
Number of reflections | 49027 | |
<I/σ(I)> | 6.8 | 2.5 |
Completeness [%] | 98.5 | 97.2 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000, potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |