1X1R
Crystal structure of M-Ras in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-25 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.899, 70.668, 43.422 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.440 - 1.300 |
| R-factor | 0.18269 |
| Rwork | 0.182 |
| R-free | 0.20003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4q21 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.293 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.064 | 0.286 |
| Number of reflections | 49027 | |
| <I/σ(I)> | 6.8 | 2.5 |
| Completeness [%] | 98.5 | 97.2 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG8000, potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
