1X1I

Crystal Structure of Xanthan Lyase (N194A) Complexed with a Product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Detector technology	CCD
Detector	RIGAKU JUPITER 210
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	53.802, 90.677, 77.920
Unit cell angles	90.00, 99.09, 90.00

Refinement procedure

Resolution	47.360 - 1.800
R-factor	0.176
Rwork	0.176
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS (1.1)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	68423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	PEG4000, Ammonium Formate, Sodium Bicine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K