1X0V
Crystal Structure of Homo Sapien Glycerol-3-Phosphate Dehydrogenase 1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9798, 0.9800, 0.900 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 113.488, 113.488, 155.436 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.207 |
Rwork | 0.205 |
R-free | 0.24900 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 88158 | |
Completeness [%] | 98.8 | 85.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.1M tri-Sodium Citrate dihydrate, 0.5M Ammonium Sulfate, 1.0M Lithium sulfate monohydorate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |