1X0L

Crystal structure of tetrameric homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	200
Detector technology	CCD
Collection date	2003-11-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	60.963, 143.243, 177.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.440 - 1.850
Rwork	0.216
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	CCP4 ((TRUNCATE))
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.850
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1