1WZ9
The 2.1 A structure of a tumour suppressing serpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.115, 95.047, 139.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.570 - 2.100 |
| R-factor | 0.20729 |
| Rwork | 0.205 |
| R-free | 0.24933 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xu8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.152 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 78.600 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.045 |
| Number of reflections | 42714 |
| <I/σ(I)> | 22.2 |
| Completeness [%] | 99.8 |
| Redundancy | 36.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | 0.1M BisTris, 2.9M (NH4)2SO4, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
