1WZ1

Crystal structure of the Fv fragment complexed with dansyl-lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2004-01-01
Detector	RIGAKU RAXIS V
Wavelength(s)	1.000
Spacegroup name	P 43 21 2
Unit cell lengths	81.630, 81.630, 74.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.600 - 1.850
Rwork	0.216
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2dlf
RMSD bond length	0.007
RMSD bond angle	1.187
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.600	1.820
High resolution limit [Å]	1.800	1.800
Rmerge	0.078	0.421
Number of reflections	23960
<I/σ(I)>	28.4
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG 8000, magnecium acetate, sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K