1WYX
The Crystal Structure of the p130Cas SH3 Domain at 1.1 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.071, 56.193, 37.924 |
| Unit cell angles | 90.00, 116.22, 90.00 |
Refinement procedure
| Resolution | 34.100 - 1.140 |
| R-factor | 0.17651 |
| Rwork | 0.176 |
| R-free | 0.19109 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.308 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.100 | 1.150 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Completeness [%] | 93.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | acetate, magnesium chloride, PEG 8000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
