1WY1
Crystal Structure of the PH0671 protein from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-03 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.188, 78.722, 52.013 |
| Unit cell angles | 90.00, 108.60, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nog |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.450 |
| Number of reflections | 45212 | |
| <I/σ(I)> | 11.9 | 1.82 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.7 | 295 | PEG3350, magnesium acetate, pH 7.7, microbatch, temperature 295K |
