1WXC
Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.664, 96.734, 54.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.200 |
| R-factor | 0.1799 |
| Rwork | 0.180 |
| R-free | 0.20830 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.028 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.085 | 0.366 |
| Number of reflections | 102888 | |
| <I/σ(I)> | 16.4 | 4.6 |
| Completeness [%] | 97.8 | 99 |
| Redundancy | 8.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | peg3350, sodium nitrate, hepes, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
