1WX5
Crystal Structure of the copper-free Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein in the monoclinic crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-09-05 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.850, 93.200, 65.590 |
| Unit cell angles | 90.00, 93.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.020 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wxc |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.680 | 2.090 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.125 | 0.278 |
| Number of reflections | 46161 | |
| <I/σ(I)> | 7.3 | 3.4 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 5.01 | 4.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | PEG4000, ammonium chloride, hepes, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
