1WWR
Crystal structure of tRNA adenosine deaminase TadA from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-15 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.176, 151.953, 54.133 |
| Unit cell angles | 90.00, 113.42, 90.00 |
Refinement procedure
| Resolution | 35.470 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1UAQ |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.176 |
| Number of reflections | 56975 | |
| <I/σ(I)> | 19.1 | 2 |
| Completeness [%] | 96.5 | 77.7 |
| Redundancy | 3.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | potassium chloride, PEG 8000, magnesium chloride, MES, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
