1WW7
Agrocybe cylindracea galectin (Ligand-free)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-02-10 |
| Detector | SIEMENS HI-STAR |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.071, 96.307, 121.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 51284 | |
| <I/σ(I)> | 11.09 | 1.86 |
| Completeness [%] | 95.4 | 85.9 |
| Redundancy | 5.32 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 293 | ammomium sulfate, pipes, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
