1WVU
Crystal structure of chitinase C from Streptomyces griseus HUT6037
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 127.850, 127.850, 127.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.470 - 2.450 |
| R-factor | 0.17494 |
| Rwork | 0.172 |
| R-free | 0.22272 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.646 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.640 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.041 | 0.125 |
| Number of reflections | 24823 | |
| <I/σ(I)> | 14 | 5.2 |
| Completeness [%] | 96.2 | 75.5 |
| Redundancy | 4.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | Sodium chloride, Sodium acetate buffer, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
