1WVT

Crystal structure of uncharacterized protein ST2180 from Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-03-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97920
Spacegroup name	P 41 21 2
Unit cell lengths	117.577, 117.577, 79.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
Rwork	0.221
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1woz
RMSD bond length	0.010
RMSD bond angle	1.294
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	25266
<I/σ(I)>	10.7
Completeness [%]	100.0	100
Redundancy	10.7	10.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	Ammonium sulfate, PEG400, HEPES buffer, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K