1WUU
crystal structure of human galactokinase complexed with MgAMPPNP and galactose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97916, 0.97931, 0.96408, 0.97000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.200, 109.600, 115.800 |
| Unit cell angles | 90.00, 95.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.25800 |
| Structure solution method | MAD |
| Data reduction software | MOSFLM |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 61550 | |
| <I/σ(I)> | 21.5 | 4.9 |
| Completeness [%] | 93.7 | 94 |
| Redundancy | 3.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 277 | PEG8000, DMSO, MgAMPPNP, galactose, NaCl, HEPPS, batch, temperature 277K |
