1WTY
Crystal structure of a probable nucleotidyl transferase protein from thermus thermophilus HB8
Replaces: 1WJ8Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-27 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.90000, 0.97899, 0.979333 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.320, 116.250, 123.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.370 - 2.200 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | CrystalClear ((MSC/RIGAKU)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.240 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.064 | 0.361 |
| Number of reflections | 153969 | |
| <I/σ(I)> | 12.3 | 3.9 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.64 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG6000, 0.05M Imidazole-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
