1WQ5
Crystal structure of tryptophan synthase alpha-subunit from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-19 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.774, 44.626, 71.716 |
| Unit cell angles | 90.00, 96.46, 90.00 |
Refinement procedure
| Resolution | 49.860 - 2.300 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1BKS_A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.860 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21699 | |
| Completeness [%] | 98.0 | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | Ammonium sulfate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
