1WPA
1.5 Angstrom crystal structure of human occludin fragment 413-522
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.020, 35.310, 107.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 20588 | |
| Completeness [%] | 97.9 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 293 | ammonium sulfate, pH 5.6, EVAPORATION, temperature 293K |
| 1 | EVAPORATION | 5.6 | 293 | ammonium sulfate, pH 5.6, EVAPORATION, temperature 293K |
