1WOV
Crystal structure of heme oxygenase-2 from Synechocystis sp. PCC 6803 in complex with heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.063 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.163, 74.585, 72.661 |
| Unit cell angles | 90.00, 108.15, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.750 |
| R-factor | 0.19818 |
| Rwork | 0.193 |
| R-free | 0.25374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dve |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.871 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 53138 | |
| Completeness [%] | 89.2 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | pottasium sodium tartrate, pottasium phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
