1WOL

Crystal Structure of ST0689, an archaeal HEPN homologue

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-02
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9765, 0.97894, 0.97924
Spacegroup name	P 31 2 1
Unit cell lengths	62.553, 62.553, 64.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.350 - 1.620
R-factor	0.187
Rwork	0.187
R-free	0.22200
Structure solution method	MAD
RMSD bond length	0.041
RMSD bond angle	3.600
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.680
High resolution limit [Å]	1.620	1.620
Completeness [%]		100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	293	tert-butanol, citrate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K