1WND
Escherichia coli YdcW gene product is a medium-chain aldehyde dehydrogenase as determined by kinetics and crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 102 |
| Detector technology | CCD |
| Collection date | 2002-10-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.036, 79.680, 158.669 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.18588 |
| Rwork | 0.184 |
| R-free | 0.21309 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.490 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 | 0.198 |
| Number of reflections | 116969 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 96.3 | 96.3 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG 4000, Imidazol malate, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
