1WMW

Crystal structure of geranulgeranyl diphosphate synthase from Thermus thermophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL40B2
Synchrotron site	SPring-8
Beamline	BL40B2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2000-02-28
Detector	RIGAKU RAXIS IV
Wavelength(s)	0.97880, 0.97777, 0.97760, 0.96860
Spacegroup name	P 43
Unit cell lengths	139.880, 139.880, 73.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.550
R-factor	0.175
Rwork	0.175
R-free	0.19800
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.039	0.115
Number of reflections	182752
Completeness [%]	89.2	81.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	296	PEG 4000, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K