1WMI
Crystal structure of archaeal RelE-RelB complex from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL40B2 |
Synchrotron site | SPring-8 |
Beamline | BL40B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794, 0.9792, 0.9000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 47.533, 142.651, 37.168 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
Rwork | 0.228 |
R-free | 0.27600 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.040 | 0.137 |
Number of reflections | 11137 | |
<I/σ(I)> | 24.3 | |
Completeness [%] | 93.1 | 72.3 |
Redundancy | 4.7 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.4 | tri-Ammonium citrate, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.4K |