1WLZ
Crystal structure of DJBP fragment which was obtained by limited proteolysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.671, 68.519, 69.208 |
Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
Resolution | 19.950 - 1.600 |
R-factor | 0.2 |
Rwork | 0.200 |
R-free | 0.22300 |
Structure solution method | MAD+SIR |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.042 | |
Number of reflections | 51566 | |
Completeness [%] | 99.4 | 95.1 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG MME 2000, 0.1M Na Acetate pH 4.6, 0.2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |