1WLS

Crystal structure of L-asparaginase I homologue protein from Pyrococcus horikoshii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-04
Detector	MARRESEARCH
Wavelength(s)	0.98000
Spacegroup name	P 21 21 2
Unit cell lengths	125.676, 77.200, 98.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.480 - 2.160
Rwork	0.211
R-free	0.25340
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.931
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.160	2.160
Number of reflections	52174
<I/σ(I)>	7.7
Completeness [%]	100.0	99.9
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Tris buffer, PEG 400, NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	Tris buffer, PEG 400, NaCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K