1WLI
L122Y mutant of FMN-binding protein from Desulfovibrio vulgaris (Miyazaki F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-07 |
| Detector | OXFORD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.673, 79.391, 39.970 |
| Unit cell angles | 90.00, 91.25, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.153 |
| Rwork | 0.153 |
| R-free | 0.18500 |
| Structure solution method | AB INITIO |
| Starting model (for MR) | 1flm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.660 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 29692 | |
| Completeness [%] | 98.0 | 84.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG6000, sodium acetate, Tris, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
